FastQCFastQC Report
Sun 14 Apr 2019
SRR6576866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076745
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52240.4851659399393543No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37890.35189390245601326No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29550.27443823746569523No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18860.17515753497810532No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17810.16540592247932426No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15660.1454383349818202No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14350.133272037483341No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13720.12742106998407235No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12700.11794807498525649No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12630.11729796748533776No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12230.11358306748580213No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12230.11358306748580213No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12040.11181848998602269No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11930.11079689248615039No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11650.10819646248647545No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11560.10736060998657992No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11430.10615326748673083No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11380.10568890498678887No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11250.10448156248693981No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10770.10002368248749705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.071.186411
GTATCAA27350.050.180521
TCAACGC27050.049.6806644
ATCAACG27500.048.867713
CAACGCA27900.048.5039255
AACGCAG28500.047.6476636
TATCAAC31600.042.83862
ACGCAGA36100.037.6165777
CGCAGAG36650.037.052078
GGTAATC5250.034.9055028
GTCTTAG11300.034.107121
GCAGAGT40750.033.3225869
CTTAGGT10600.032.3597873
GTACATG46200.032.2497941
TACATGG46300.031.6740382
AAGGGTA6300.031.3254575
TTAGGTA11050.031.0419654
ACATGGG47300.030.9943183
GTAATCA6300.030.578199
TAGGTAT11200.030.2066885
Produced by FastQC (version 0.11.2)