FastQCFastQC Report
Sun 14 Apr 2019
SRR6576865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157804
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53990.46631381477348494No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35530.30687404776628857No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30170.26057951086712433No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17010.14691605833111648No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16870.14570687266583982No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13960.12057308490901741No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13960.12057308490901741No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13210.11409530455932092No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12520.10813574663760014No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12420.10727204259097395No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12170.10511278247440844No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11750.10148522547857841No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11700.10105337345526531No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11700.10105337345526531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.066.806371
GTATCAA26050.052.835311
TCAACGC30700.043.7899674
CAACGCA31350.042.5803265
ATCAACG31600.042.5427863
AACGCAG32600.040.9476476
TATCAAC34950.038.59952
GTCTTAG11350.034.053921
ACGCAGA40100.033.2847987
TAGGTAT11050.032.7534185
TTAGGTA11350.031.8890674
CTTAGGT11350.031.8890673
TCTTAGG13350.030.9847742
CGCAGAG42650.030.853968
GTACATG51750.030.7861581
AGGTATA11950.030.2866346
GTACCGT1252.3617758E-730.08216
TACATGG53300.029.8964372
ACATGGG53500.029.4332333
GCAGAGT46100.028.9527199
Produced by FastQC (version 0.11.2)