FastQCFastQC Report
Sun 14 Apr 2019
SRR6576860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154157
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44900.38902852904760793No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25580.22163362523469513No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23050.1997128640210994No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15390.13334407710562773No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14980.12979170078247587No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14860.1287519808830168No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12840.11125002924212218No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12770.1106435259674377No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12770.1106435259674377No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12280.10639800304464643No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12240.10605142974482674No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG12060.10449184989563812No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12040.10431856324572826No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12030.10423191992077335No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11640.1008528302475313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.071.768851
GTATCAA27500.055.726561
TCAACGC32800.046.4131974
ATCAACG33150.045.923173
CAACGCA34200.044.650635
AACGCAG34450.044.3266076
TATCAAC39650.038.887322
ACGCAGA41400.036.8853037
CGCAGAG42700.035.7623338
CTTAGGT8250.033.6022533
TAGGTAT8300.033.3998345
GCAGAGT46150.033.29259
TTAGGTA8650.032.5915834
GTCTTAG9650.031.1766911
ACAACGC804.7891214E-429.3663753
AGGTATA9600.029.3663756
CTAAGAC15650.029.1224373
ACCTAAG15000.028.5185531
TCTTAGG10550.028.5170672
GGTATAG9800.028.287617
Produced by FastQC (version 0.11.2)