FastQCFastQC Report
Sun 14 Apr 2019
SRR6576859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149025
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44810.3899828115141098No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23750.20669698222405952No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21620.1881595265551228No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15270.13289528078153215No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14860.1293270381410326No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14820.12897891690781316No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12890.11218206740497379No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12850.11183394617175432No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12410.10800461260634016No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12310.10713430952329149No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12220.10635103674854769No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11840.10304388503296273No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11690.10173843040838973No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11630.10121624855856051No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG11560.10060703640042644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.066.406921
GTATCAA26300.054.3395231
TCAACGC31450.044.83174
ATCAACG31550.044.5406343
CAACGCA33000.042.725975
AACGCAG33600.041.9630056
TATCAAC38250.036.6254352
ACGCAGA40800.034.557777
CGCAGAG42100.033.490668
TAGGTAT7300.032.8346145
GTCTTAG8550.032.4402281
ACCTAAG14450.031.2321131
GCAGAGT45750.030.9214619
GTACATG51000.030.7874891
CTTAGGT7650.030.7180143
TACATGG51300.030.6074472
ACATGGG52900.029.4962643
CCTAAGA16450.029.4353562
TAAGACA17450.029.357274
CTAAGAC16850.028.7290923
Produced by FastQC (version 0.11.2)