FastQCFastQC Report
Sun 14 Apr 2019
SRR6576856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961040
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37190.3869766086739366No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23570.2452551402647132No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21380.22246732706234915No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14250.14827686672771165No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13590.1414093065845334No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11000.11445933571963705No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10950.11393906601182052No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10290.1070715058686423No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9970.10374177973861651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5750.061.504541
GTATCAA20700.050.1148071
TCAACGC25050.040.5238954
ATCAACG25550.039.916883
AAGGGTA2950.039.827515
CAACGCA25950.039.1184435
AACGCAG26450.038.3789636
TATCAAC29200.035.2491842
GGTAATC3000.034.4640738
GTACATG41800.031.5860611
TACATGG43150.030.8243122
ACGCAGA32800.030.8056097
TAGGTAT7100.030.448415
CTTAAGT2950.030.2704833
ACATGGG43750.030.1867263
TATAAGC1102.968316E-629.9083962
AGGTATA7250.029.8184456
CGCAGAG34200.029.544568
CTTAGGT7200.029.3743153
GTCTTAG8300.028.4057081
Produced by FastQC (version 0.11.2)