FastQCFastQC Report
Sun 14 Apr 2019
SRR6576855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943357
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA144121.5277355232430563No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC99901.0589840325560735No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC74540.7901568547220194No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG55160.5847203126706008No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC49000.5194215975500261No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC42900.4547589088754311No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT40140.42550169236036833No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37440.3968805022912853No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA37420.39666849347595873No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA36040.38203988521842736No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT34930.37027339596780434No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC33880.3591429331631609No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA32700.346634413058895No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT32520.3447263337209561No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC32360.3430302631983438No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT28410.30115852217135186No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT26120.2768835128164629No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG25700.27243132769460554No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT25660.27200731006395246No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA25260.2677671337574216No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT24120.2556826312838088No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA23220.2461422345941144No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG22590.23946395691132838No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA21480.2276974676607053No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG21070.22335128694651124No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA21070.22335128694651124No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20800.2204891679396029No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT20000.21200881532654128No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG19140.2028924362675No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC18730.1985462555533059No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG18590.1970621938460201No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA18450.19557813213873432No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC18370.19473009687742815No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG18070.19154996464753005No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA17960.19038391616323405No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG17810.188793850048285No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA17540.1859317310413767No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG17270.1830696120344684No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA17130.1815855503271826No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA17070.18094952388120297No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT16840.17851142250494775No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA16710.17713336520532524No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA16610.17607332112869253No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT16550.1754372946827129No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC16540.17533129027504965No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG16480.17469526382907002No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC16080.17045508752253918No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT15910.1686530125922636No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC15910.1686530125922636No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG15790.16738095970030434No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA15110.16017265997920194No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC15010.15911261590256923No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT14790.1567805189339773No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG14450.15317636907342608No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT14400.15264634703510974No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT14090.14936021039754832No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC13950.14787614869026255No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA13850.14681610461362982No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA13580.1439539856067215No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13550.1436359723837317No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA13290.14087985778448667No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA13280.1407738533768234No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT13190.13981981370785398No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG12900.1367456858856191No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC12670.1343075845093639No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT12450.13197548754077193No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC12320.13059743024114942No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA12280.13017341261049634No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA12130.12858334649554728No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT12100.12826533327255746No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA12100.12826533327255746No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT12020.12741729801125132No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC11940.12656926274994515No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG11920.1263572539346186No Hit
CTCTTACAGTATGGACTTTAAGAACTAAGGAATTCTCCAAGACATGGATT11430.12116303795911834No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT11340.1202089982901489No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC11300.11978498065949583No Hit
ACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA11210.11883094099052638No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC11120.11787690132155695No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG11110.11777089691389368No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC10980.11639283961427116No Hit
ATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGT10980.11639283961427116No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC10940.11596882198361808No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA10880.11533279553763845No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT10760.11406074264567921No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT10750.11395473823801593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.11268268534605667No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC10620.11257668093839343No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG10540.11172864567708725No Hit
TCCCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTC10380.11003257515447493No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT10330.10950255311615857No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG10250.1086545178548524No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC10160.10770047818588298No Hit
GGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACA10050.106534429701587No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTTGTGCA9980.1057923988479441No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG9890.10483835917897467No Hit
GTCTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAA9890.10483835917897467No Hit
TCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTG9870.10462635036364812No Hit
ATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGA9600.10176423135673981No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC9450.10017416524179075No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC9440.10006816083412749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.071.364051
GTGCAAG8000.046.995841
TGCAAGG7750.044.8758242
GTACTAG857.932613E-944.2313771
TATCAAC26750.044.099372
ATCAACG27000.043.6817743
CAACGCA27150.043.613515
AAGGGTA7650.043.610125
TCAACGC27300.043.373884
AACGCAG28300.041.6751946
GTATCAA30650.040.1726261
GCAAGGG8750.039.738733
GTCTTAA2250.039.685371
TAGGTAT26550.038.5817835
AGGGTAA8900.038.013066
TTAGGTA26750.037.942014
GGTAATC9000.037.590698
CTTAGGT27150.037.3830073
GTCTTAG28300.037.1981161
ACGCAGA32450.036.345397
Produced by FastQC (version 0.11.2)