FastQCFastQC Report
Sun 14 Apr 2019
SRR6576850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384175
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57300.41396499720049845No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35310.25509780193978365No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28920.20893311900590603No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17550.12679032636769194No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17040.12310582115700688No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16470.11898784474506474No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15190.10974045911824734No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14630.10569472790651473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.064.501081
GTATCAA27950.055.9891051
TCAACGC34050.044.5884974
ATCAACG34650.043.6807483
CAACGCA35650.042.455485
AACGCAG36750.041.3126036
TATCAAC39900.038.1688882
ACGCAGA44700.033.645957
CGCAGAG46650.032.2383658
GTACATG54850.032.0537951
TAGGTAT10550.031.6332115
TACATGG55900.031.3642142
CTTAGGT11100.030.4892653
ACATGGG57900.030.1996423
TTAGGTA11100.030.0658044
GCAGAGT51450.029.4133959
GTATAGG3850.029.383111
AAGGGTA5150.029.2065415
GTCTTAG12400.028.8894441
ACCTAAG19050.028.7018761
Produced by FastQC (version 0.11.2)