FastQCFastQC Report
Sun 14 Apr 2019
SRR6576840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280977
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49570.3869702578578694No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32380.25277581096303836No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26530.20710754369516393No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16890.1318524844708375No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15300.11944008362367162No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14650.11436583170501892No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14060.10975997227116491No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13760.10741800984717133No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13540.10570057073624273No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13480.10523217825144401No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13470.10515411283731091No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13120.10242182334265174No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12970.10125084213065497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.064.131761
GTATCAA23950.052.0630341
TCAACGC28300.043.1750834
ATCAACG28850.042.514883
CAACGCA29850.040.9331635
AACGCAG31100.039.4375046
TATCAAC36000.034.725012
GTCTTAG11000.031.6539651
ACGCAGA38950.031.3686077
CGCAGAG40350.030.4168728
GTACATG51050.030.4163631
CTTAGGT10200.030.408153
TACATGG51550.030.0848942
TAGGTAT10550.029.399355
ACATGGG52750.029.2211743
TTAGGTA10800.029.1539444
GGTATAG10800.028.7176917
AGGTATA11050.028.067976
GCAGAGT44250.027.7360679
ACCTAAG15950.027.730411
Produced by FastQC (version 0.11.2)