FastQCFastQC Report
Sun 14 Apr 2019
SRR6576839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321770
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA48030.36337638167003333No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30850.23339915416449156No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24790.187551540737042No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16210.12263858311203917No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14540.1100040097747717No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14110.10675079628074476No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13820.10455676857547076No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13810.10448111244770271No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13730.10387586342555816No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13520.10228708474242872No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13390.10130355508144381No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13320.10077396218706733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.069.401551
GTATCAA24800.051.322511
TCAACGC29150.042.5722924
ATCAACG29400.042.210283
TACGGTA450.001013278441.7839162
CAACGCA30350.041.1988035
CTTAGGT9850.039.1326523
AACGCAG32200.038.831796
TAGGTAT9450.038.799355
GTCTTAG10950.038.3152541
AGGTATA10000.037.1354566
TTAGGTA10550.035.6450464
GGTATAG10650.035.3036737
TATCAAC36200.034.281282
TCTTAGG13150.033.601892
TATAGTA11950.033.429559
GTATAGT12300.032.4783028
TAGAGCG1052.0633088E-631.3379365
ACGCAGA40900.030.6808557
GTACATG53850.030.551711
Produced by FastQC (version 0.11.2)