FastQCFastQC Report
Sun 14 Apr 2019
SRR6576839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321770
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA46900.35482723923224163No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30760.23271824901457897No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27140.20533073076253813No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18640.14102302215967982No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16230.1227898953675753No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15770.11930971349024413No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15740.11908274510693993No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15660.1184774960847954No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15080.11408944067424741No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14630.11068491492468432No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14420.10909613624155488No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14020.10606989113083215No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13650.10327061440341359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.060.7599681
GTATCAA26100.054.7538071
TCAACGC30650.044.774944
ATCAACG31500.043.715933
CAACGCA32100.042.8988085
AACGCAG34300.040.28436
TATCAAC40250.034.4538152
ACGCAGA41800.033.056267
GTCTTAG11850.032.132751
GGTAATC4700.031.9988828
TAGGTAT9750.031.8142725
ACCTAAG16150.031.4363771
CGCAGAG44650.030.9462838
GTACATG54800.030.7960591
TTAGGTA10250.030.7208774
CTTAGGT10250.030.7208773
TACATGG54900.030.1405752
AGGTATA10450.029.683176
AAGGGTA5550.029.6386015
CTAAGAC18350.029.1978873
Produced by FastQC (version 0.11.2)