FastQCFastQC Report
Sun 14 Apr 2019
SRR6576823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA71510.7037741624044256No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC48250.47485810846054444No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32800.32280509756488823No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG26460.26040923419411416No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25750.2534216848260937No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC21870.21523620377268618No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA18620.18325094258104327No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA18560.1826604454513514No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT17870.1758697284598949No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC17130.16858693052702853No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT16770.16504394774887732No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16180.15923739264024062No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15320.15077360044799049No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT14930.14693536910499336No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14640.14408129964481597No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14550.1431955539502782No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT13550.13335393512208035No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT12530.1233154839173186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.12302023535247265No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12320.12124874396339705No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA12280.12085507921026914No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG11510.11327703271255682No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT11150.10973404993440561No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG10770.10599423477969044No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG10550.10382907863748692No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA10480.10314016531951307No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA10170.10008926348277175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.068.4920961
GTATCAA30350.052.03781
TCAACGC35100.044.1747554
ATCAACG35150.044.111923
CAACGCA36150.043.0216455
TATCAAC36700.042.263442
AACGCAG37100.041.9200136
ACGCGTG500.001704448337.5887037
AAGGGTA5550.035.556885
TTAGGTA12850.035.102294
CTTAGGT13000.034.6972663
ACGCAGA44700.034.6875577
ACCTAAG21800.034.0673941
CGCAGAG45800.033.8544548
TAGGTAT13350.033.4356425
GCAGAGT47350.032.9446959
GGTAATC6050.032.6182988
CTAAGAC23350.032.5983353
GTCTTAG14300.032.5414921
ACTTAAG4550.032.0249631
Produced by FastQC (version 0.11.2)