FastQCFastQC Report
Sun 14 Apr 2019
SRR6576819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321420
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA56400.42681357933132535No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35710.2702395907432913No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29760.22521227164716745No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19360.1465090584371358No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19180.14514688743926987No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16130.12206565664209715No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14990.1134385736556129No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14550.11010882232749618No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14500.10973044149475565No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14160.10715745183211998No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14010.10602230933389838No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14000.10594663316735027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.10019524450969412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.064.140641
GTATCAA33550.056.7357371
TCAACGC37600.049.613314
ATCAACG38200.048.834053
CAACGCA38550.048.390685
AACGCAG39950.046.9301266
TATCAAC44200.042.428852
ACGCAGA49150.037.9544377
TAGACCG500.001704089637.5911455
CGCAGAG50700.036.7940948
GCAGAGT55450.033.7256779
GTACATG60500.031.0741181
CATATAG1701.2369128E-1030.4046043
CTTAGGT11750.030.3928413
TACATGG60950.030.2289452
TTAGGTA12050.029.6361714
GTCTTAG13050.029.5323181
ACATGGG62850.029.3073393
TAGGTAT12250.029.1523195
GCGCGAC1005.334054E-528.1922939
Produced by FastQC (version 0.11.2)