FastQCFastQC Report
Sun 14 Apr 2019
SRR6576818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380831
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA56320.40787033315445553No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34120.2470975810942831No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28430.20589051085904067No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17460.12644559689056808No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16810.12173828658249995No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16330.11826211897038813No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15480.11210640549060676No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14040.10167790265427122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.063.448111
GTATCAA29050.053.5104521
TCAACGC34800.043.8949434
ATCAACG34900.043.50143
CAACGCA36050.042.633695
AACGCAG36700.041.4943856
TATCAAC41200.037.5339972
ACGCAGA45000.033.735317
GGTAATC5200.033.4420628
GTACATG56700.032.815941
CGCAGAG46950.032.534378
TACATGG57800.032.1214682
ACATGGG59600.030.9147643
GTAATCA7150.030.2364449
GCAGAGT51100.030.075019
GTGCAAG7400.029.9183041
AAGGGTA5850.028.9239125
AGGGTAA5900.028.6787956
CTAAGAC20800.026.8911743
ACCTAAG19400.026.7092551
Produced by FastQC (version 0.11.2)