FastQCFastQC Report
Sun 14 Apr 2019
SRR6576818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380831
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53840.38991013382521106No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35260.2553534791730487No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29390.21284284608326437No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20020.14498515748849786No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18850.13651199893397525No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16530.11971052214210139No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15430.11174430469767843No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15080.10920959914718022No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14550.10537133074214006No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14210.1029090453502275No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14070.10189516313002822No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13960.10109854138558594No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13880.10051918011690063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.066.102191
GTATCAA31850.052.104721
TCAACGC36600.044.3020634
ATCAACG36650.044.2416273
CAACGCA37750.043.0769655
AACGCAG38700.042.140976
TATCAAC44650.036.430612
ACGCAGA48800.033.322867
CGCAGAG50050.032.5845228
GTACATG61800.031.4936981
TAGGTAT11050.031.4742535
TACATGG61750.031.0623992
GTCTTAG11900.030.8148061
GGTAATC5750.030.2426538
GCAGAGT54500.030.010199
TTAGGTA11450.029.9642454
ACCTAAG19050.029.8608551
ACATGGG63700.029.8076613
CTTAGGT11250.029.6614113
CTAAGAC20250.029.4757373
Produced by FastQC (version 0.11.2)