FastQCFastQC Report
Sun 14 Apr 2019
SRR6576816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369735
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51050.37269982879900126No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33050.24128754832139065No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26670.19470919557432642No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18560.13550066253691406No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16690.12184838673174009No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16170.11805203196238688No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15260.11140841111601878No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14480.10571387896198899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.062.6985131
GTATCAA28900.059.06461
TCAACGC34650.048.0154154
ATCAACG34800.047.8084533
CAACGCA36350.045.640565
AACGCAG37800.044.1384666
TATCAAC40750.041.1963842
ACGCAGA44600.037.3034557
CTTAGGT10300.035.134533
CGCAGAG47700.034.8791248
GGTAATC4600.034.7377558
GTCTTAG11200.034.4282041
TTAGGTA10550.034.301964
TAGGTAT10850.032.9203535
GCAGAGT51350.032.3998839
ACTTAAG4650.032.3605231
CTTAAGA4900.031.6691472
GTACATG57550.031.3764951
TCTTAGG13350.031.3492582
TACATGG57650.031.0773662
Produced by FastQC (version 0.11.2)