FastQCFastQC Report
Sun 14 Apr 2019
SRR6576812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38370.4365838367265201No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29650.33736540940686266No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27550.3134710633780461No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15330.17442872601036105No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14190.16145750959471775No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12600.14336607617289948No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT11760.13380833776137285No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11720.13335320736082396No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11710.13323942476068676No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10850.12345412114888567No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10640.12106468654600401No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9840.11196207853502627No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9730.11071046993351684No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9670.11002777433269349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.10820725273049796No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9500.10809347013036072No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC9390.10684186152885129No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9350.1063867311283024No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9260.1053626877270674No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9060.10308703572432297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.066.530821
GTATCAA22250.051.5581471
TCAACGC23650.046.292984
ATCAACG24200.045.2408643
CAACGCA24850.043.8684125
AACGCAG26000.042.1088036
TATCAAC27300.039.9542352
GGTAATC4000.035.239278
ACGCAGA31800.034.426627
GTAATCA4600.032.6856969
TTAGGTA9750.032.289444
CGCAGAG34000.032.1990178
AGGTATA10100.032.100966
GTGCAAG5300.031.9347951
TAGGTAT9900.031.8002075
GTACATG37650.031.4682831
GTCTTAG10950.030.914051
TACATGG38600.030.8156052
GGTATAG10550.030.2846167
CTTAGGT10350.029.9635943
Produced by FastQC (version 0.11.2)