FastQCFastQC Report
Sun 14 Apr 2019
SRR6576811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953946
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40050.4198350850048116No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30350.3181521805217486No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27530.288590758806054No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15040.15766091581703787No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13480.1413077889104834No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13340.13984020059835672No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12120.12705121673553849No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12060.12642225031605564No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11810.12380155690154368No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10780.11300430003375454No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10010.10493256431705777No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9910.103884286951253No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9730.1019973876928044No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9690.10157807674648249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.10094911032699964No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9540.10000566069777535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.065.347951
GTATCAA22200.046.0924571
TCAACGC25100.039.8834154
CAACGCA25600.039.4716225
ATCAACG25300.039.3844343
AACGCAG26100.038.715466
TTAAGTA2250.035.510184
TATCAAC28800.034.4349332
GGTAATC3400.033.1738628
CTTAGGT10750.031.9171143
TAGGTAT10350.031.7866065
GTACATG40750.031.7063331
GTCTTAG11850.031.4363271
ACGCAGA32750.031.2830377
TTAGGTA10800.030.8973374
TACATGG42100.030.7015192
AGGTATA10900.030.1826976
TCTTAGG13300.030.0383832
ACATGGG43550.029.3555343
CGCAGAG34750.029.2120918
Produced by FastQC (version 0.11.2)