FastQCFastQC Report
Sun 14 Apr 2019
SRR6576802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312115
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6240.19992630921295035No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4220.13520657449978374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.11374012783749579No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3490.11181775947967897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG250.005235760556.3910694
GTATCAA8850.040.899281
CGGTCGT250.001702756737.5940492-93
TAGGTCA1351.3225872E-831.3283675
ATCAACG11450.031.1915633
AACGCAG11450.031.1915636
TCCGACC2000.030.5451588
TCAACGC11700.030.5250764
CAACGCA11750.030.395185
GTACATG15900.029.2688921
GATATAG1303.3137803E-728.9277611
CCGACCA1952.0008883E-1128.9184939
TACATGG16400.028.6631772
CATGGGG7000.028.195534
ACATGGG16500.027.9107273
CCGCAAT350.00883291126.8571980-81
ATACAGC1057.412551E-526.8528866
ATGGGGC1956.020855E-1026.508625
ACGCAGA13300.026.4995577
GGTATCA5000.026.3242631
Produced by FastQC (version 0.11.2)