FastQCFastQC Report
Sun 14 Apr 2019
SRR6576797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136963
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44630.3925369603056564No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31800.27969247899887684No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23850.2097693592491576No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15400.1354485590120347No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15040.132282229061104No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14750.12973157437840985No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13360.11750602262342749No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13130.11548308959922178No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12810.11266857408728341No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11960.10519251725869708No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11910.1047527492099567No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11650.10246595535650677No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11420.10044302233230104No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11380.10009120789330876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.062.8995061
GTATCAA23650.051.080021
TCAACGC27600.042.7327584
ATCAACG27700.042.5784873
CAACGCA28300.041.6739275
AACGCAG29550.039.911076
TATCAAC34400.034.4342962
TAGGTAT8300.032.8342485
TTAGGTA8800.032.5719224
GTACATG47600.031.8966161
ACGCAGA38100.031.0779767
TACATGG48350.030.9157452
CGCAGAG38950.030.5204018
CTTAGGT8800.030.436063
ACATGGG49050.029.889033
AGGTATA9100.029.431386
ATTATCC15100.028.9402483
GTCTTAG9800.028.7789041
GCAGAGT41500.028.5318269
ACCTAAG15050.028.4219571
Produced by FastQC (version 0.11.2)