FastQCFastQC Report
Sun 14 Apr 2019
SRR6576793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923484
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57590.6236166517232568No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29920.32399045354332073No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22780.24667454985684645No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20650.22360972144617558No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19630.21256459234810782No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15760.17065807312308603No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14780.16004608634258957No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13270.1436949638542736No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12600.13643983003495458No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12450.13481554634406226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.12983440969199248No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG10950.1185727094351391No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10790.11684014016485396No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10770.11662356900606831No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10670.1155407132121401No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10380.11240043140974831No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10020.10850215055160674No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT9510.10297958600257286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.060.6041221
GTATCAA26700.052.1126751
TCAACGC30550.044.602744
ATCAACG31150.043.5927773
CAACGCA31350.043.4645545
AACGCAG32450.042.1359756
GGTAATC4500.041.7659388
TATCAAC33400.040.9552352
TAGGTAT9950.034.472645
CGCAGAG39750.034.279598
ACGCAGA39900.034.150727
AAGGGTA5250.034.0094075
CTTAGGT10000.033.830413
GTGCAAG6500.032.543341
GCAGAGT42050.032.4046069
GTCTTAG11450.031.201181
TTAGGTA10900.031.0370734
ACCTAAG18800.031.0046441
CTAAGAC19400.031.0015223
GTACATG48850.030.6002861
Produced by FastQC (version 0.11.2)