FastQCFastQC Report
Sun 14 Apr 2019
SRR6576792_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576792_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916930
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57740.6297100105787792No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27860.3038399877853271No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22930.2500736152159925No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18400.2006696258165836No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17760.19368981274470243No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG17150.1870371784105657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.15846356864755218No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA13810.15061127894168586No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13380.14592171703401569No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12810.13970532101687153No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC12600.1374150698526605No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11350.12378262244664259No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10850.11832964348423543No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10580.11538503484453558No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10240.11167700915009869No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA9830.10720556640092484No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9790.10676932808393225No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9400.10251600449325467No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9340.1018616470177658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.071.0712361
GTATCAA27300.055.387611
TCAACGC33050.045.7937474
ATCAACG33000.045.72073
CAACGCA33600.045.184045
AACGCAG34450.043.932766
TATCAAC35200.042.59612
GTGCAAG5850.037.845321
AAGGGTA4800.037.2103845
ACGCAGA41800.036.091357
CGCAGAG42000.036.031398
GGTAATC5100.033.1746838
GCAGAGT45950.033.03639
TATAGGG1001.4101443E-632.9018142
GTACTAG753.2323058E-431.4035661
GTAATCA6850.030.8742459
AGGGTAA5800.030.79486
GTACATG50050.030.5878891
TACATGG51750.029.5185382
ACCTAAG19000.029.0069791
Produced by FastQC (version 0.11.2)