FastQCFastQC Report
Sun 14 Apr 2019
SRR6576791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423434
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8360.19743336623889435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.1438240670328788No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5480.12941804389822262No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4730.11170572037200602No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4470.10556544821625094No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4300.10155065488364184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.049.588361
GTACATG22800.036.517451
ATCAACG13750.035.5452963
TACATGG22950.035.425412
TCAACGC13900.034.823624
CAACGCA14000.034.5748835
AACGCAG14050.034.4518366
ACATGGG23550.034.1237453
TATCAAC15350.032.7587172
CTAATAG600.004160214731.3299873
ACGCAGA16600.029.1595367
ATGGGGG5050.028.8484045
CATGGGG13350.028.1617874
CGCAGAG17450.027.7391598
GGGTACC4550.026.8542777
TGGGTAC4600.026.5623826
CATGGGT6400.026.4346774
CTATAGA909.4612926E-426.1330131
ATATACG2900.025.9282656
TACGCTA2950.025.4888069
Produced by FastQC (version 0.11.2)