FastQCFastQC Report
Sun 14 Apr 2019
SRR6576791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423434
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9280.2191604830977201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.12469475762456486No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5150.12162462154668734No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4650.10981640586254292No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4590.1083994199804456No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4490.1060377768436167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.050.723171
GTACATG21400.036.0645451
GGGTACC3650.036.05387
TGGGTAC3550.035.745496
TCAACGC11550.035.4016234
TACATGG22050.035.2148362
GGTATCA3950.034.5502551
CAACGCA11900.034.3603975
ATCAACG12200.033.515473
ATGGGTA4550.033.054035
ACATGGG23050.033.0316243
AACGCAG12800.031.944436
TATCAAC13900.030.1318342
GGTACCT4400.029.9082648
ATAGCAC804.7766164E-429.3741873
CATGGGT7150.028.922284
CGCAAGT350.00883592526.856476-77
ACCTAAG1606.8099325E-826.4711531
CATGGGG13450.026.2074554
ACGCAGA15850.025.7973967
Produced by FastQC (version 0.11.2)