FastQCFastQC Report
Sun 14 Apr 2019
SRR6576790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476459
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21220.14372224355705102No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20980.14209673279109003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20910.14162262548435142No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20290.13742338933895218No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19870.13457874549852045No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19200.13004086127687936No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18670.1264511916687155No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18390.12455476244176099No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18260.12367427744353213No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18100.12259060359955812No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT17570.11900093399139428No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17270.11696904553394304No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16820.11392121284776618No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC16330.11060246170059582No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15840.10728371055342546No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15420.10443906671299373No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG15000.10159442287256198No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA14930.10112031556582338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27800.051.8982961
CAACGCA34750.040.1580775
ATCAACG35150.039.7010883
AACGCAG39500.036.0426566
TCAACGC39600.035.477034
ACCTAAG7800.033.13811
GTACTAG5550.033.0241131
GGTATCA11750.032.7971041
ACGCAGA43700.031.933487
CGCAGAG45350.030.875238
TAGGTAT4500.030.2800625
CCTAAGA8900.030.0974462
TATCAAC48050.029.8298632
CGTTAAC2051.8189894E-1229.8022461
TACATGG57350.029.4994492
GTACATG58000.029.4129221
ACATGGG59400.028.6347483
GCAGAGT49700.028.0783319
ATGGGGG17200.027.5907925
CATGGGG36750.027.4885854
Produced by FastQC (version 0.11.2)