FastQCFastQC Report
Sun 14 Apr 2019
SRR6576788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751435
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23330.31047262903644357No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13830.1840478551039012No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11920.15862982160799005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14000.055.3972131
GGTATCA4150.047.5700041
TCAACGC18000.043.594474
ATCAACG18200.042.599063
CAACGCA18750.041.600095
AACGCAG19400.040.4484836
ATGCGTA308.795377E-539.1893184-85
TATCAAC20700.037.2421232
CTTAGGT3650.036.045633
ACGCAGA23400.032.93187
TAGGTAT4250.032.062445
TTAGGTA4350.031.3253694
CGCAGAG24800.031.0727448
GTCTTAG5600.031.0560111
GGTAATC1859.094947E-1230.4787398
CTAAGAC10350.028.6014253
TACATGG29500.028.5227832
GTACATG29850.028.1864681
ACCTAAG9350.028.1519451
ACATGGG30950.027.934743
Produced by FastQC (version 0.11.2)