FastQCFastQC Report
Sun 14 Apr 2019
SRR6576787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945637
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31620.3343777792112618No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20160.2131896277324174No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15220.16094970903211275No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10880.11505471972860623No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10080.1065948138662087No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10010.10585457210324893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.058.653841
GTATCAA19700.052.1881521
ATCAACG22600.047.00873
TCAACGC22650.046.6973884
CAACGCA23700.044.8268625
AACGCAG24050.043.976716
TATCAAC25900.040.6561742
TATAGGG906.179762E-736.5623252
GGTAATC2950.035.0480658
ACGCAGA31200.033.891547
CGCAGAG31450.033.7715648
GTACATG38150.031.1521261
TACATGG38700.030.7317852
GCAGAGT35450.029.8283829
ACATGGG39450.029.7900523
ACCTAAG10750.029.3932781
CCCTATA804.7750722E-429.3804362
ATTATCC10950.029.192623
CTAAGAC11900.028.442243
GTAATCA4350.028.0897279
Produced by FastQC (version 0.11.2)