FastQCFastQC Report
Sun 14 Apr 2019
SRR6576787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945637
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27480.2905977663733547No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15870.16782338254531073No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14000.14804835259195653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.059.1050341
GTATCAA18900.051.741781
GTCGTAT450.001012234841.791441
TCAACGC23400.041.5751234
ATCAACG23350.041.060323
CAACGCA24000.040.5357445
CTTAGGT5250.040.283973
AACGCAG24400.039.8712236
TAGGTAT5250.038.4935725
TATCAAC26700.036.0979422
GTCTTAG6700.035.0860941
AGGTATA5950.034.7547956
TTAGGTA5800.034.0330124
GGTATAG6250.032.33467
ACGCAGA31750.030.493177
GTATAGT7350.030.0531228
CGCAGAG33200.029.4445068
TATAGTA7700.028.687079
TCTTAGG7600.028.4566672
GTACATG38500.028.3313371
Produced by FastQC (version 0.11.2)