FastQCFastQC Report
Sun 14 Apr 2019
SRR6576783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283323
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9390.33142385192871743No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6470.22836126964630474No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4980.17577111635836132No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4910.1733004380159747No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4690.16553544893990252No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4150.14647593029863443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.13976980336929934No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3810.13447549263561376No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3750.13235776834213955No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3620.1277693657062787No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3600.12706345760845394No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3580.12635754951062922No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3550.1252986873638921No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3440.12141619282585599No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3380.11929846853238177No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3330.11753369828781991No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3280.11576892804325806No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3210.11329824970087143No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3210.11329824970087143No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3080.1087098470650106No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3010.10623916872262401No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2930.10341553633132503No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG2890.10200372013567553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.00522521656.418432
TGAACCG250.00523252456.39851894
ATAAGAC601.6011145E-646.9987643
GTATCAA9550.045.7845951
TAACGCC450.001012791841.776684
AACGCAG12100.035.346186
ATGGGAG2750.034.180925
ATAACGC550.002713919634.180923
ATCAACG12600.033.943553
TCAACGC12600.033.943554
CAACGCA12650.033.8093875
CAAACGC702.1766825E-433.58242
ATGGGTA3550.033.097725
TACATGG19800.033.0057332
GTACATG20350.032.344721
ACATGGG20500.031.6381913
TACTATA600.00414909731.3435752
CATGGGA6150.031.332514
ATGGGCG1052.0585358E-631.332515
CTAATAG600.004156274731.332513
Produced by FastQC (version 0.11.2)