FastQCFastQC Report
Sun 14 Apr 2019
SRR6576773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553683
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13550.24472486964562754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.20481033371080562No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9470.17103649561211018No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8350.15080831450487012No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8300.14990527070543974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7760.14015239767159188No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6740.12173030416321251No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6490.11721508516606073No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6390.11540899756720001No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6330.11432534500788356No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6200.11197743112936463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6130.11071316981016213No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5810.10493368949380784No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5740.10366942817460532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14600.061.880531
TACGGGT250.005236600556.3945854
AACGCAG21250.042.0232856
CAACGCA21500.041.530895
TCAACGC21550.041.4345324
ATCAACG21850.040.865643
GGTATCA6000.039.996741
ACGCAGA24950.035.9732557
GTACATG30750.035.19561
CGCAGAG25750.034.698228
TACATGG31400.034.2768752
TATCAAC26450.034.117012
ACATGGG32050.032.992153
GCAGAGT29300.030.4941679
CATGGGG12000.028.9805494
CTAGTAC2053.6379788E-1127.5095523
CCTAGTA2253.6379788E-1227.1553972
CATGGGT8700.027.00894
AGATCGG700.00883238326.856996
CATGGGA15650.026.1252864
Produced by FastQC (version 0.11.2)