FastQCFastQC Report
Sun 14 Apr 2019
SRR6576772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586097
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14850.2533710290276183No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10150.17317952489093102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.16669595647136906No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9360.15970052738710488No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8970.1530463387459755No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8350.14246788500879545No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7210.12301717975010962No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6860.11704547199524994No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6520.11124438446195765No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6490.11073252379725541No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6480.11056190357568799No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6180.10544329692866539No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5950.10151903183261474No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG5930.1011777913894799No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5880.10032469028164279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.058.353511
AACGCAG21150.045.1101266
ATCAACG20850.045.08683
TCAACGC21150.044.8917434
CAACGCA21350.044.6913645
ACGCAGA24850.038.3935287
GGTATCA5700.037.1107861
CGCAGAG25750.036.86918
GTACATG32900.036.862631
TATCAAC26350.036.3925132
TACATGG34150.035.3756942
ACATGGG35050.034.0620773
GCAGAGT28800.032.964569
CCGCTAA753.272378E-431.3353234
CATGGGG13500.030.9871524
GGTACCT5300.029.2635148
ATGGGTA6100.028.510015
TATACAG2650.028.3791625
GGGTACC5150.028.2906487
TGGGTAC5500.028.1993876
Produced by FastQC (version 0.11.2)