FastQCFastQC Report
Sun 14 Apr 2019
SRR6576770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464506
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11140.23982467395469595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.2133449298824988No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8680.1868651858103017No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7540.16232298398728973No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7290.15694092218399763No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6520.14036417182985797No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6350.13670436980361933No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6290.1354126749708292No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6240.13433626261017081No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5870.1263708111412985No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5630.12120403181013809No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5190.11173160303634398No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4790.10312030415107662No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4770.10268973920681326No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4680.10075219695762809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12650.052.1873931
GGTATCA4950.039.057711
AACGCAG17100.038.206916
ATCAACG17250.038.1471563
CAACGCA17300.037.765215
TCAACGC17450.037.709944
GTCTTAT1054.955109E-835.9276471
TACATGG26250.035.6325572
GTACATG26050.035.4794161
ACATGGG26650.034.392253
GTATACG550.00267184934.2945751
CATGGGA12300.031.7172994
ACGCAGA20450.031.4850047
ATGGGCG1052.060924E-631.3351635
TATCAAC21900.030.2620412
CATGGGT7250.029.8224324
CGCAGAG21600.029.8087168
CATGGGC4450.027.4622784
CGGTATC1755.5315468E-926.8587112
AATTCGC350.00883728926.85581878-79
Produced by FastQC (version 0.11.2)