FastQCFastQC Report
Sun 14 Apr 2019
SRR6576770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464506
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12130.2611376386957327No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9110.19612233211196411No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8010.17244126017747888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.17244126017747888No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7280.15672563971186593No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7190.1547880974626808No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5990.1289542008068787No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5890.12680137608556186No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5750.12378742147571829No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5670.12206516169866481No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5380.11582197000684598No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5270.11345386281339745No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5150.11087047314781726No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5120.11022462573142221No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5000.10764123606584199No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4990.1074259535937103No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4790.10312030415107662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.063.6003951
TGCCCGC405.667858E-447.008991
ATCAACG18200.046.48243
GGTATCA4550.045.4592441
CAACGCA18700.045.239555
TCAACGC18750.045.1189164
AACGCAG18950.044.6427236
CCCGCTA450.001013451141.776773
AACCGTC500.001701184537.5990947
TAACGCA500.001701184537.5990944
TATCAAC23000.037.4028052
ACGCAGA23250.036.3862237
CGCAGAG23450.035.875478
CCGCTAA550.002715670334.1809964
CGCTAAA550.002715670334.1809965
GTACATG27950.032.6287771
GCAGAGT26200.032.28939
TACATGG28400.031.946252
TATAACG600.004154560631.3393292
ACATGGG29600.030.3269733
Produced by FastQC (version 0.11.2)