FastQCFastQC Report
Sun 14 Apr 2019
SRR6576765_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576765_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349914
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10020.2863560760644044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.23891584789405398No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6570.1877604211320496No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6540.18690306761089867No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5710.16318295352572346No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5460.15603834084946588No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5290.15118000422961073No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4850.13860548591939734No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4760.1360334253559446No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4750.1357476408488943No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4510.128888812679687No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4430.12660253662328458No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4250.1214584154963791No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4130.11802900141177546No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4030.11517115634127242No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3900.11145595774961847No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3870.11059860422846758No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3850.11002703521436696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3790.10831232817206514No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3780.10802654366501482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.10802654366501482No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3680.10516869859451179No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3570.10202506901695846No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3530.10088193098875724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.005179108656.54771
GTATCAA14900.048.3881341
AACGCAG16800.042.7991456
TCAACGC18100.039.465534
CAACGCA18450.038.7168665
ATCAACG18350.038.671753
TAGGTAT1950.038.5601165
GGATAAG500.001678737.6984671
CGCAGAG20000.036.4212388
ACGCAGA20200.035.595337
GTGTAAT1204.083631E-935.342311
CTTAGGT2000.035.2463573
GTCTTAG2450.034.621041
GTACATG20450.033.6428831
GGTATAG2100.033.567967
TATTTAG702.1832349E-433.5679552
GCAGAGT21900.033.2614029
TACATGG20800.033.2129142
AAGGGTA851.7525505E-533.1730425
GGGTACC3450.032.6922727
Produced by FastQC (version 0.11.2)