FastQCFastQC Report
Sun 14 Apr 2019
SRR6576762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239126
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5050.21118573471726204No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3240.13549342187800573No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2460.10287463512959694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5400.060.9327051
GTACATG15100.038.600321
ATCAACG8550.038.4677163
CAACGCA8700.037.804485
CGCGCGT500.001701897637.588467
TCAACGC8750.037.5884554
TACATGG15400.037.543142
ACATGGG16000.036.7074783
GGTATCA2100.035.8135071
AACGCAG9450.035.301336
CTCTATT801.15393905E-535.253922
TAACGCC959.431751E-734.620954
GTACTAC550.002711245334.185621
ACAGCGG550.00271681434.171328
CATGGGT4650.032.3341564
TATCAAC10250.032.1011352
ATGGGTA2800.031.8830685
ATGTAGC753.2737383E-431.3237136
CATGGGA8850.030.7928054
CGCAGAG10850.030.313278
Produced by FastQC (version 0.11.2)