FastQCFastQC Report
Sun 14 Apr 2019
SRR6576757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316484
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9360.2957495481604125No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5910.18673929803718356No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4900.1548261523489339No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4700.1485067175591815No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4570.14439908494584244No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4140.13081230014787476No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4040.12765258275299857No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3730.11785745882888234No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3500.11059010882066708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.10964219360220422No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3350.10585053272835276No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3280.10363873055193945No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3270.10332275881245181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.051.5477451
GGTATCA3250.039.1325341
ATCAACG10950.037.336383
AACGCAG11450.035.7059676
TCAACGC11500.035.5507244
CAACGCA11550.035.3968245
GTACATG19800.034.7332341
TACATGG20250.034.577082
GGTTAGC702.1754351E-433.587188
GTAGTAC702.1835303E-433.5659563
ACATGGG20750.032.8380173
TATAGGT753.2733503E-431.3282244
CATGGGT5600.031.0485084
CGCAGAG13200.030.6355828
ACGCAGA13400.030.1592627
TATCAAC13600.030.0612752
GTATCAT953.687723E-529.7498821
CATGGGG8100.029.5877674
ATATAGT700.00883354626.8527644
GGACCGT700.00883354626.8527646
Produced by FastQC (version 0.11.2)