FastQCFastQC Report
Sun 14 Apr 2019
SRR6576756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342940
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8740.25485507668979995No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6380.18603837405960227No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5370.15658715810345833No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5160.15046363795416107No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4730.13792500145798098No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4390.12801073074007116No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4080.11897124861491806No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3690.10759899690908031No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3530.10293345774771098No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3440.10030909196944071No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3430.10001749577185513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.054.7393761
AACGCAG9800.040.7622076
ATCAACG9700.040.6979373
TCAACGC9900.039.875764
GTACATG19900.039.838051
CAACGCA9950.039.6753775
TAGTACA952.1109372E-839.5759434
TACATGG19850.039.30182
ACATGGG20500.038.0556453
ACGCAGA12350.031.9605227
CGCAGAG12500.031.952918
TATCAAC12850.031.8185942
CATGGGG8350.031.518564
GTACAGG1901.2732926E-1129.8036581
CATGGGA10300.029.2016644
ATGGGTA3850.026.8551035
GCAGAGT15350.026.0202859
CATGGGT5650.025.785654
ATGGGAG4800.025.45645
GTACATT1351.4544237E-524.3685176
Produced by FastQC (version 0.11.2)