FastQCFastQC Report
Sun 14 Apr 2019
SRR6576755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268678
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5740.21363863062848465No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4080.15185463640491592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.14627174536061754No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3140.11686851919397942No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3100.11537974824883318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.041.5315061
TACATGG18900.040.2954522
GGTACCT2450.040.2729538
ACATGGG18950.039.6708073
GTACATG19500.039.2966771
TGGGTAC2650.037.2334826
ATACCGT651.4132405E-436.1423956
GGGTACC2900.035.6438757
CATGGGG6600.034.8828854
CATGGGT4400.034.170994
ATGGGTA3300.034.1709865
GTACCTG2950.033.447039
CATGGGA9450.031.8206024
ATCAACG11300.030.3532143
TCAACGC11450.029.9555724
AACGCAG11500.029.825336
GTAATAG804.764495E-429.3821011
ATGGGGA3550.029.1175355
CAACGCA11800.029.067065
TGCCCGC700.00881379126.8636341
Produced by FastQC (version 0.11.2)