FastQCFastQC Report
Sun 14 Apr 2019
SRR6576754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291604
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.18586850660484766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.17455178941303962No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4040.1385440528936503No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3360.1152247568620458No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3270.11213837944609813No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3120.10699441708618537No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2960.10150752390227842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.052.6787381
ATCAACG11650.043.1658063
CAACGCA12100.041.1720545
TCAACGC12100.041.1720544
AACGCAG12400.040.1759536
TATCAAC13200.037.7410472
GTACATG20300.036.2411961
ACATGGG20700.036.1001323
TACATGG20600.036.047232
ACGCAGA14400.034.5959637
TATAGAG550.002714185334.1805735
GGTATCA4100.033.3570821
CGCAGAG15400.032.6546558
ATGGGAC2250.031.332195
GCAGAGT16400.030.6635159
CATGGGT4600.030.6510564
CATGGGA12000.030.5488854
CATGGGG5850.029.7254124
ATGGGAG5600.029.3739285
GCTTGCG350.00883408926.85616382-83
Produced by FastQC (version 0.11.2)