FastQCFastQC Report
Sun 14 Apr 2019
SRR6576747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291493
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3920.13448007327791747No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3210.1101227130668661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.049.5572621
GACATAT901.3023964E-841.79831
CAACGCA10350.039.506315
ATCAACG10300.039.2417873
TCAACGC10600.038.574564
AACGCAG11000.037.1718446
TATCAAC12150.034.4448932
ACGCAGA12250.032.9951367
CATATAG1151.13261194E-732.6948783
CCATACC902.5877573E-531.332594
CGCAGAG13150.031.0943188
ACATATA1252.3428584E-730.0947742
GTACATG15450.029.5225851
ACATGGG16050.028.9899673
TACATGG16600.027.4773452
GCAGAGT14700.027.1762249
ACTATAT700.008826422526.8565048
CTCGGTA350.00883353726.85650442-43
CCTAAGA909.4733795E-426.1239382
TGCTATA909.4733795E-426.1239382
Produced by FastQC (version 0.11.2)