FastQCFastQC Report
Sun 14 Apr 2019
SRR6576744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117771
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2760.23435310899966882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.14689524585848807No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1470.12481850370634537No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1250.10613818342376308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGC200.002157719870.5035194
GGTACCT1103.8198777E-1142.7112548
GTACATG10300.039.2280041
ACATGGG10350.039.0384983
TACATGG10550.038.7437632
ATGGGAG2350.037.9857565
ATGGGAC906.145656E-736.5418475
TTAGGCT651.4089057E-436.1402934
GTATCAA4550.035.107711
CATGGGA6050.034.945574
CTATGGT550.00271214434.1690034
TGGGTAC1457.3305273E-1032.401646
AACGCAG4950.032.2707256
CATGGGT2400.031.3215874
TGTTAGA600.004153586531.3215872
CATGGGC1658.54925E-1131.3215854
TCAACGC5200.030.7192484
ATCAACG5250.030.4266833
CAACGCA5300.030.1396395
ATGGGAT1901.2732926E-1129.6730795
Produced by FastQC (version 0.11.2)