FastQCFastQC Report
Sun 14 Apr 2019
SRR6576743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127428
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.19697397746178233No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2320.18206359669774302No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1570.1232068305239037No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1340.10515742223059295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA4800.046.05661
GTACATG10300.039.2732161
TACATGG10100.037.6927532
GTACATC500.001689888237.629221
AACGCAG5650.037.4333156
ACATGGG10050.036.9449653
ATCAACG6000.036.0330353
TCAACGC6000.036.0330354
CAACGCA5900.035.847165
CGCAGAG6150.034.3899548
CGCGGTA550.002708102634.18153494
ACGCAGA6250.033.839727
ATGGGAT1457.3123374E-1032.413525
CATGGGT1605.820766E-1132.312234
TATCAAC6900.032.0142252
CTTAATA600.004147405331.3330752
ATGGGAC1351.3078534E-831.333075
CATGGGA6650.031.0974854
GCAGAGT7050.029.999759
ATGGGGG953.72665E-529.6839625
Produced by FastQC (version 0.11.2)