FastQCFastQC Report
Sun 14 Apr 2019
SRR6576743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127428
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2820.22130144081363595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.1585208902282073No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1790.14047148193489656No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1500.1177135323476787No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1310.10280315158363938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAG502.9942683E-547.02
GTACATG12000.039.1666681
TACATGG11850.038.86922
ACATGGG12150.037.9094663
TCCTATA500.001696353837.62
GTATCAA5050.036.2970281
CATGGGG4000.035.254
ATGGGAC959.3633025E-734.6315775
CATGGGA6850.034.306574
TGGGATA550.002707992234.1818166
GTATCCA702.1741592E-433.571431
ATGGGAG3250.033.2615365
ATGGGAT1850.033.0270275
ATGGGGG1501.0513759E-931.3333345
GTAGCCC600.00414723631.3333343
GCAATCC902.5760062E-531.3333329
TGCAATC1052.0463776E-631.3333328
ATAATTG953.7264377E-529.684213
TACGCTA953.7264377E-529.684219
TAATTGC953.7264377E-529.684214
Produced by FastQC (version 0.11.2)