FastQCFastQC Report
Sun 14 Apr 2019
SRR6576741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372726
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9260.24843987272151663No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5200.1395126715066832No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4790.12851263394557932No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4710.1263662851531688No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4620.123951642761707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.10597597162526898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.005233272456.3999982
GTATCAA10100.043.2772251
GCCGTAA250.001701643737.662-63
AACGCAG12650.034.1818166
TACATGG23400.034.1452982
GTACATG23450.034.0724951
ATCAACG12800.033.4140623
ACATGGG23900.033.037663
TCAACGC13200.032.4015124
CAACGCA13250.032.2792435
ACGCAGA14800.029.2162177
CGCAGAG14900.029.0201348
CATGGGT6500.028.9230754
TATCAAC15550.028.4115772
TATAGAG1005.314021E-528.1999992
CTTAGAC1057.408357E-526.8571433
CATGGGA11250.026.737784
ATGGGAG5400.025.2407385
GGTATCA5500.024.7818181
ATGGGTA4000.024.6755
Produced by FastQC (version 0.11.2)