FastQCFastQC Report
Sun 14 Apr 2019
SRR6576740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229375
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6880.29994550408719345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.17395095367847413No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3520.15346049046321525No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3350.1460490463215259No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3310.14430517711171664No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3280.14299727520435968No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3150.13732970027247957No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2650.11553133514986376No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2610.11378746594005448No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2610.11378746594005448No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2550.11117166212534059No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2480.10811989100817439No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2430.1059400544959128No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2430.1059400544959128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA200.002162994170.481694
GTATCAA10050.046.754021
TACATGG21600.040.896782
GTACATG21650.040.3682671
ACATGGG22000.039.939623
GTATTGG608.8154826E-539.1564941
CATGGGT4750.038.5794534
ATGGGTA3250.037.5902375
AACGCAG12450.037.363796
GGGTACC3300.037.0206837
GGTACCT3250.036.144468
ATCAACG12900.036.06043
TCAACGC13300.034.9758764
CATGGGG6600.034.8848764
CAACGCA13650.034.079065
CATGGGA12750.033.9049154
TATCAAC14150.033.206922
ATGGGAC2000.032.8914575
ATGGGAG4600.032.6871575
ACGCAGA14400.032.3041087
Produced by FastQC (version 0.11.2)