FastQCFastQC Report
Sun 14 Apr 2019
SRR6576739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247045
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6110.24732336214050074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.1781052035054342No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4000.16191382136857657No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3690.14936550021251188No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3400.1376267481632901No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3210.1299358416482827No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3190.1291262725414398No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2810.11374445951142505No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2810.11374445951142505No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2780.11253010585116072No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2750.1113157521908964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.10200570746220325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8650.044.643651
CCTCTAT608.7013235E-539.2446751
TACATGG21650.038.6406172
GTACATG21700.038.629781
ACATGGG22800.036.6916433
ATGGGTA2400.035.248715
GTCCTAC550.00268655234.2498971
GTATAGG550.00268655234.2498971
CATGGGT4000.034.073754
ATGGGAG4700.033.9987565
CATGGGA12500.033.4627724
CATGGGG7550.031.747184
GTACTAG753.2302295E-431.3957421
ACTATAC600.004155461731.332193
AATTCGC300.00415945131.3321978-79
ATACCGT753.26886E-431.3321886
AACGCAG12300.029.4216866
ATCAACG12600.029.0941733
GTAAGTA650.006146045428.9220182
TCAACGC12750.028.7518884
Produced by FastQC (version 0.11.2)