FastQCFastQC Report
Sun 14 Apr 2019
SRR6576739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247045
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6800.2752534963265802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.1611042522617337No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3880.15705640672751928No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3410.13803153271671154No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3310.13398368718249712No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3200.12953105709486126No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3180.12872148798801838No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3140.12710234977433263No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2900.11738752049221801No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2800.11333967495800361No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2770.11212532129773929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.045.9160731
TACATGG21850.038.5110632
GTACATG22000.038.248491
ACATGGG21600.037.8609473
CTAATAG651.4103157E-436.153693
ATCAACG11300.034.9379043
TCAACGC11500.034.3302884
CATGGGA12100.034.181674
CAACGCA11550.034.181675
AACGCAG11550.034.181676
GTATATC550.002712956634.181674
ATGGGAT3550.033.098455
CATGGGG6950.031.7840354
GCATAGG753.264361E-431.3395441
TCTATAC600.004154804631.3331993
ATGGGAC2100.031.3331975
TATCAAC13100.030.8610762
CATGGGC3050.030.8195364
ATGGGAG5550.030.4863535
TATATAC1102.9561252E-629.9089623
Produced by FastQC (version 0.11.2)