FastQCFastQC Report
Sun 14 Apr 2019
SRR6576737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386377
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8300.21481609930197706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.1568416339481905No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5060.13096017620096434No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4700.12164285141196293No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4610.11931352021471257No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4450.11517248697515639No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3920.10145531436912654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12700.049.971431
TACATGG29550.039.7717132
GTACATG30150.039.1361541
ACATGGG30550.038.4598963
ATCAACG16400.038.4008033
ATGGGAC3150.037.299995
AACGCAG17050.037.212496
CAACGCA17150.036.9955065
CATGGGA15850.036.768154
TCAACGC17300.036.6747324
GGTACCT3300.035.604548
CATGGGT7050.033.998554
CGTACGG352.1848899E-433.56999684-85
ATGGGAG6500.033.260125
GGGTACC3550.033.097187
TATCAAC19700.032.2150882
GGTATCA6050.031.0810991
ATGGGTA4400.030.975955
ACGCAGA20500.030.9498987
CGCAGAG20950.030.28518
Produced by FastQC (version 0.11.2)