FastQCFastQC Report
Sun 14 Apr 2019
SRR6576733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170013
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5120.3011534412074371No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3320.1952791845329475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.18880909106950644No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.18586813949521508No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3030.1782216654020575No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT2380.1399892949362696No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2260.13293101115797026No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC2210.12999005958367887No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2130.12528453706481268No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2110.12410815643509614No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2040.1199908242310882No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1950.11469711139736373No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1840.1082270179339227No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1770.10410968572991477No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1720.10116873415562339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC250.00523363956.3857044
GTATAAG450.001009849241.7917751
GTATCAA6500.040.5058751
CATGGGT2700.038.2865874
CTATATC651.4056553E-436.1659552
GTACATG13350.035.9221421
TACATGG13350.035.9221422
ATGGGAG2650.035.4627085
ACATGGG13600.034.5500643
AACGCAG7550.034.2297336
GTATAAC550.002706078834.1932681
ACGCAGA8150.031.709757
CAACGCA8200.031.5164035
TCAACGC8200.031.5164034
ATACTCG300.004108401531.40853146-47
GTACAGA902.575136E-531.343831
ATATGTG600.0041562631.3253925
ATCAACG8250.031.3253923
GTTACAC600.0041562631.3253923
CACTCTA1201.641929E-731.3253929
Produced by FastQC (version 0.11.2)