FastQCFastQC Report
Sun 14 Apr 2019
SRR6576732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189154
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5430.28706768030282204No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3710.1961364813855377No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3380.17869037926768666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.16230161667212958No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2760.14591285407657253No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT2590.13692546813707349No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2330.12318005434725145No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2290.12106537530266344No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC2220.11736468697463441No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2170.11472133816889943No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG2090.11049198007972341No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2070.1094346405574294No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2020.10679129175169438No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2010.1062626219905474No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1940.10256193366251837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA301.3682533E-462.6663179
TGATCGC250.00522941656.399689
ACTTTAT1100.051.2859951
GTATCAA6200.049.2869451
ACATGGG13600.037.6689073
TACATGG14350.036.364812
GTACATG14250.036.290091
AACGCAG8650.034.7743726
GTCATAA550.002707860434.1906661
GGGTAGA550.002707860434.1906661
ATATAGG550.002711374334.1816253
TATTCTG1800.033.9442525
ATGGGAC1256.208211E-933.8398065
TTTATTC1950.033.74343
CATGGGT2650.033.697924
CATGGGA7600.033.394554
CAACGCA9100.033.054765
ATCAACG9150.032.874133
TCAACGC9150.032.874134
CGCAGAG9500.032.1577158
Produced by FastQC (version 0.11.2)