FastQCFastQC Report
Sun 14 Apr 2019
SRR6576730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387627
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8570.22108883024144343No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5410.1395671612142601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.13621342166567346No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5090.1313118023254314No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4920.12692614291574117No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3980.10267602617980687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG352.9358143E-453.7115556
GTATCAA11300.047.425761
ACTATCG405.6734553E-446.9976165
CGCTAAA503.0066089E-546.9976125
TATCGGT555.278174E-542.72517
CCCGCTA608.815897E-539.1646773
AACGCAG13800.038.483556
TCAACGC14100.037.3314364
ATCAACG14250.037.2682843
CAACGCA14050.037.1297875
GTACATG24200.036.7142221
TACATGG24650.035.4718552
ACATGGG24550.035.0328453
CCGCTAA702.1829932E-433.569724
CGCAGAG16850.032.0755238
ACGCAGA16900.031.9806257
TATCAAC17650.030.096892
GGTACCT3300.029.9075728
GGGTACC3350.029.461197
ATCGGTC804.7765247E-429.373518
Produced by FastQC (version 0.11.2)